She married Anand Shah on 19 February 1999. She has two daughters Ameyaa (16 November 2000) and Keia (9 November 2002).She has acted in Malayalam, Hindi, Tamil, Telugu and Kannada films. She
is best remembered for her portrayals in Maniratnam's Roja (1992) and
Shankar's Gentleman (1993). She was named after the late Hindi actress
Madhubala. Madhoo is the niece of actress Hema Malini. She did her
schooling from St.Joseph's high school in Juhu – Mumbai.